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clone x75  (Novus Biologicals)


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    Structured Review

    Novus Biologicals clone x75
    Clone X75, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clone+x75/CA125%2FMUC16+Antibody+(X75)/pm37794035-452-18-20
    Average 94 stars, based on 7 article reviews
    clone x75 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE
    Article Snippet: .. Both cell lysates and supernatants were boiled for 5 min at 95 °C and 30 μL of sample was loaded onto a 4–12% Criterion XT BisTris gel (Bio-Rad, 3450124) which was run in MOPS XT buffer (Bio-Rad, 1610788) at 180 V for 90 min. Proteins were transferred to a 0.2 μm nitrocellulose membrane (Bio-Rad, 1620112) using the Trans-Blot Turbo Transfer System (Bio-Rad), at a constant 2.5 A for 15 min. Total protein was quantified using Revert 700 stain (LI-COR Biosciences, 926-11011) before a 1:1000 dilution of primary antibody (clone X75, Novus Biologicals, NB600-1468) was incubated overnight at 4 °C. .. A 1:15,0000 IR800 dye-labeled secondary antibody (LI-COR, 926-32210) was used according to manufacturer’s instructions for visualization on a LI-COR Odyssey instrument.

    Staining:

    Article Title: Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE
    Article Snippet: .. Both cell lysates and supernatants were boiled for 5 min at 95 °C and 30 μL of sample was loaded onto a 4–12% Criterion XT BisTris gel (Bio-Rad, 3450124) which was run in MOPS XT buffer (Bio-Rad, 1610788) at 180 V for 90 min. Proteins were transferred to a 0.2 μm nitrocellulose membrane (Bio-Rad, 1620112) using the Trans-Blot Turbo Transfer System (Bio-Rad), at a constant 2.5 A for 15 min. Total protein was quantified using Revert 700 stain (LI-COR Biosciences, 926-11011) before a 1:1000 dilution of primary antibody (clone X75, Novus Biologicals, NB600-1468) was incubated overnight at 4 °C. .. A 1:15,0000 IR800 dye-labeled secondary antibody (LI-COR, 926-32210) was used according to manufacturer’s instructions for visualization on a LI-COR Odyssey instrument.

    Incubation:

    Article Title: Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE
    Article Snippet: .. Both cell lysates and supernatants were boiled for 5 min at 95 °C and 30 μL of sample was loaded onto a 4–12% Criterion XT BisTris gel (Bio-Rad, 3450124) which was run in MOPS XT buffer (Bio-Rad, 1610788) at 180 V for 90 min. Proteins were transferred to a 0.2 μm nitrocellulose membrane (Bio-Rad, 1620112) using the Trans-Blot Turbo Transfer System (Bio-Rad), at a constant 2.5 A for 15 min. Total protein was quantified using Revert 700 stain (LI-COR Biosciences, 926-11011) before a 1:1000 dilution of primary antibody (clone X75, Novus Biologicals, NB600-1468) was incubated overnight at 4 °C. .. A 1:15,0000 IR800 dye-labeled secondary antibody (LI-COR, 926-32210) was used according to manufacturer’s instructions for visualization on a LI-COR Odyssey instrument.

    other:

    Article Title: Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE.
    Article Snippet: Total protein was quantified using Revert 700 stain (LI-COR Biosciences, 926-11011) before a 1:1000 dilution of primary antibody (clone X75, Novus Biologicals, NB600-1468) was incubated overnight at 4 °C.



    Similar Products

    anti-ca125 antibody clone x75  (Thermo Fisher)
    90
    Thermo Fisher anti-ca125 antibody clone x75
    MUC16 expression, CAR design and functionality in vitro. (A) Left —Gene expression of MUC16 , MSLN , MUC1 and FOLR1 in patient samples across clinical stages. Data were taken from a TCGA OC cohort of 375 patients. The color scale varies from the low expression (blue) to high expression (red). The different clinical stages are shown in a graphical colorful legend as indicated. Right —Volcano plot where the x- axis is the fold change in gene expression and y -axis is the statistical significance (-log10 p value). Genes with a high degree of significance (p values <0.05) and substantial fold changes are in red and genes with lower significance or smaller fold changes are in green. Non-significant genes are in gray. (B) Representation of MUC16 protein at the plasma membrane and recognition sites of the antibodies used in this study. K-series hybridoma supernatants staining of HEK, HeLa, and OVCAR3 cell lines. The commercial <t>anti-CA125</t> antibody, <t>X75,</t> was used as a positive control, and staining with the secondary only (anti-mouse) was used as a background control. Samples were then analyzed by flow cytometry. (C) Expression of MUC16CAR constructs in J76-NFAT-GFP assessed by staining with biotinylated anti-mFab antibody and fluorescent streptavidin. Samples were then analyzed by flow cytometry. Left —CAR expression in J76-NFAT-GFP as percent mFab-positive cells (N=4–5 independent transductions, expression of CARs, data are mean±SD). Right —Representative staining of J76-NFAT-GFP transduced or not (Mock) with the indicated MUC16CAR construct. (D) J76-NFAT-GFP cells expressing the indicated MUC16CAR construct were co-cultured (E:T=1:2) overnight with MUC16 pos (HeLa, OVCAR3) or MUC16 neg (HEK) cell lines, with effector-only (E-only) condition used as a baseline. CAR reactivity was assessed on the basis of GFP expression, N=3 independent experiments, data are mean±SD. (E) As in (C) using primary T cells from N=3–4 healthy donors. (F) Functional activity of primary T cells incubated with the indicated target cells (top: HEK, middle: HeLa, bottom: OVCAR3). Left— Degranulation activity: CAR-transduced T cells were co-cultured with target cells at E:T=1:2 overnight, in the presence of anti-CD107a fluorescent antibody. Target cells were gated out and CD107a was detected on T cells. Data are mean±SD, n=2–4 donors, N=4–6 independent experiments, one-way ANOVA, *p<0.05, **p<0.01. Right —Cytotoxic activity: CAR T cells were tested in bioluminescence-based citotoxicity co-cultures at different E:T ratios, as indicated. Anti-CD19 CAR, FMC63, was included as an irrelevant CAR control. The time point displayed is at 10 hours of co-culture. Data are mean±SD, N=3 independent experiments, two-way ANOVAs with Dunnett’s multiple comparisons, **p<0.01, ***p<0.001. (G) Cytotoxic assay in three-dimensional (3D) cultures (spheroids). Left —Representation of the assay. Right —HeLa and HEK cells were established as spheroids for 5 days. T cells were added, and killing was measured 24 hours later (day 6). Annexin V was recorded in IncuCyte live-cell imaging, and fold increase relative to target cells alone was calculated. Data are mean of normalized values±SD, N=2 different donors, one-way ANOVA, *p<0.05. ANOVA, analysis of variance; bioluminescence-based citotoxicity, bioluminescence-based cytotoxicity; CAR, chimeric antigen receptor; E:T, effector:target ratio; HEK, human embryonic kidney; OC, ovarian cancer; TCGA, The Cancer Genome Atlas.
    Anti Ca125 Antibody Clone X75, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clone+x75/oregon+green+488/pmc11015285-76-7-11
    Average 90 stars, based on 1 article reviews
    anti-ca125 antibody clone x75 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    clone x75  (Novus Biologicals)
    94
    Novus Biologicals clone x75
    MUC16 expression, CAR design and functionality in vitro. (A) Left —Gene expression of MUC16 , MSLN , MUC1 and FOLR1 in patient samples across clinical stages. Data were taken from a TCGA OC cohort of 375 patients. The color scale varies from the low expression (blue) to high expression (red). The different clinical stages are shown in a graphical colorful legend as indicated. Right —Volcano plot where the x- axis is the fold change in gene expression and y -axis is the statistical significance (-log10 p value). Genes with a high degree of significance (p values <0.05) and substantial fold changes are in red and genes with lower significance or smaller fold changes are in green. Non-significant genes are in gray. (B) Representation of MUC16 protein at the plasma membrane and recognition sites of the antibodies used in this study. K-series hybridoma supernatants staining of HEK, HeLa, and OVCAR3 cell lines. The commercial <t>anti-CA125</t> antibody, <t>X75,</t> was used as a positive control, and staining with the secondary only (anti-mouse) was used as a background control. Samples were then analyzed by flow cytometry. (C) Expression of MUC16CAR constructs in J76-NFAT-GFP assessed by staining with biotinylated anti-mFab antibody and fluorescent streptavidin. Samples were then analyzed by flow cytometry. Left —CAR expression in J76-NFAT-GFP as percent mFab-positive cells (N=4–5 independent transductions, expression of CARs, data are mean±SD). Right —Representative staining of J76-NFAT-GFP transduced or not (Mock) with the indicated MUC16CAR construct. (D) J76-NFAT-GFP cells expressing the indicated MUC16CAR construct were co-cultured (E:T=1:2) overnight with MUC16 pos (HeLa, OVCAR3) or MUC16 neg (HEK) cell lines, with effector-only (E-only) condition used as a baseline. CAR reactivity was assessed on the basis of GFP expression, N=3 independent experiments, data are mean±SD. (E) As in (C) using primary T cells from N=3–4 healthy donors. (F) Functional activity of primary T cells incubated with the indicated target cells (top: HEK, middle: HeLa, bottom: OVCAR3). Left— Degranulation activity: CAR-transduced T cells were co-cultured with target cells at E:T=1:2 overnight, in the presence of anti-CD107a fluorescent antibody. Target cells were gated out and CD107a was detected on T cells. Data are mean±SD, n=2–4 donors, N=4–6 independent experiments, one-way ANOVA, *p<0.05, **p<0.01. Right —Cytotoxic activity: CAR T cells were tested in bioluminescence-based citotoxicity co-cultures at different E:T ratios, as indicated. Anti-CD19 CAR, FMC63, was included as an irrelevant CAR control. The time point displayed is at 10 hours of co-culture. Data are mean±SD, N=3 independent experiments, two-way ANOVAs with Dunnett’s multiple comparisons, **p<0.01, ***p<0.001. (G) Cytotoxic assay in three-dimensional (3D) cultures (spheroids). Left —Representation of the assay. Right —HeLa and HEK cells were established as spheroids for 5 days. T cells were added, and killing was measured 24 hours later (day 6). Annexin V was recorded in IncuCyte live-cell imaging, and fold increase relative to target cells alone was calculated. Data are mean of normalized values±SD, N=2 different donors, one-way ANOVA, *p<0.05. ANOVA, analysis of variance; bioluminescence-based citotoxicity, bioluminescence-based cytotoxicity; CAR, chimeric antigen receptor; E:T, effector:target ratio; HEK, human embryonic kidney; OC, ovarian cancer; TCGA, The Cancer Genome Atlas.
    Clone X75, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clone+x75/CA125%2FMUC16+Antibody+(X75)/pm37794035-452-18-20
    Average 94 stars, based on 1 article reviews
    clone x75 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    MUC16 expression, CAR design and functionality in vitro. (A) Left —Gene expression of MUC16 , MSLN , MUC1 and FOLR1 in patient samples across clinical stages. Data were taken from a TCGA OC cohort of 375 patients. The color scale varies from the low expression (blue) to high expression (red). The different clinical stages are shown in a graphical colorful legend as indicated. Right —Volcano plot where the x- axis is the fold change in gene expression and y -axis is the statistical significance (-log10 p value). Genes with a high degree of significance (p values <0.05) and substantial fold changes are in red and genes with lower significance or smaller fold changes are in green. Non-significant genes are in gray. (B) Representation of MUC16 protein at the plasma membrane and recognition sites of the antibodies used in this study. K-series hybridoma supernatants staining of HEK, HeLa, and OVCAR3 cell lines. The commercial anti-CA125 antibody, X75, was used as a positive control, and staining with the secondary only (anti-mouse) was used as a background control. Samples were then analyzed by flow cytometry. (C) Expression of MUC16CAR constructs in J76-NFAT-GFP assessed by staining with biotinylated anti-mFab antibody and fluorescent streptavidin. Samples were then analyzed by flow cytometry. Left —CAR expression in J76-NFAT-GFP as percent mFab-positive cells (N=4–5 independent transductions, expression of CARs, data are mean±SD). Right —Representative staining of J76-NFAT-GFP transduced or not (Mock) with the indicated MUC16CAR construct. (D) J76-NFAT-GFP cells expressing the indicated MUC16CAR construct were co-cultured (E:T=1:2) overnight with MUC16 pos (HeLa, OVCAR3) or MUC16 neg (HEK) cell lines, with effector-only (E-only) condition used as a baseline. CAR reactivity was assessed on the basis of GFP expression, N=3 independent experiments, data are mean±SD. (E) As in (C) using primary T cells from N=3–4 healthy donors. (F) Functional activity of primary T cells incubated with the indicated target cells (top: HEK, middle: HeLa, bottom: OVCAR3). Left— Degranulation activity: CAR-transduced T cells were co-cultured with target cells at E:T=1:2 overnight, in the presence of anti-CD107a fluorescent antibody. Target cells were gated out and CD107a was detected on T cells. Data are mean±SD, n=2–4 donors, N=4–6 independent experiments, one-way ANOVA, *p<0.05, **p<0.01. Right —Cytotoxic activity: CAR T cells were tested in bioluminescence-based citotoxicity co-cultures at different E:T ratios, as indicated. Anti-CD19 CAR, FMC63, was included as an irrelevant CAR control. The time point displayed is at 10 hours of co-culture. Data are mean±SD, N=3 independent experiments, two-way ANOVAs with Dunnett’s multiple comparisons, **p<0.01, ***p<0.001. (G) Cytotoxic assay in three-dimensional (3D) cultures (spheroids). Left —Representation of the assay. Right —HeLa and HEK cells were established as spheroids for 5 days. T cells were added, and killing was measured 24 hours later (day 6). Annexin V was recorded in IncuCyte live-cell imaging, and fold increase relative to target cells alone was calculated. Data are mean of normalized values±SD, N=2 different donors, one-way ANOVA, *p<0.05. ANOVA, analysis of variance; bioluminescence-based citotoxicity, bioluminescence-based cytotoxicity; CAR, chimeric antigen receptor; E:T, effector:target ratio; HEK, human embryonic kidney; OC, ovarian cancer; TCGA, The Cancer Genome Atlas.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Efficient CAR T cell targeting of the CA125 extracellular repeat domain of MUC16

    doi: 10.1136/jitc-2023-008179

    Figure Lengend Snippet: MUC16 expression, CAR design and functionality in vitro. (A) Left —Gene expression of MUC16 , MSLN , MUC1 and FOLR1 in patient samples across clinical stages. Data were taken from a TCGA OC cohort of 375 patients. The color scale varies from the low expression (blue) to high expression (red). The different clinical stages are shown in a graphical colorful legend as indicated. Right —Volcano plot where the x- axis is the fold change in gene expression and y -axis is the statistical significance (-log10 p value). Genes with a high degree of significance (p values <0.05) and substantial fold changes are in red and genes with lower significance or smaller fold changes are in green. Non-significant genes are in gray. (B) Representation of MUC16 protein at the plasma membrane and recognition sites of the antibodies used in this study. K-series hybridoma supernatants staining of HEK, HeLa, and OVCAR3 cell lines. The commercial anti-CA125 antibody, X75, was used as a positive control, and staining with the secondary only (anti-mouse) was used as a background control. Samples were then analyzed by flow cytometry. (C) Expression of MUC16CAR constructs in J76-NFAT-GFP assessed by staining with biotinylated anti-mFab antibody and fluorescent streptavidin. Samples were then analyzed by flow cytometry. Left —CAR expression in J76-NFAT-GFP as percent mFab-positive cells (N=4–5 independent transductions, expression of CARs, data are mean±SD). Right —Representative staining of J76-NFAT-GFP transduced or not (Mock) with the indicated MUC16CAR construct. (D) J76-NFAT-GFP cells expressing the indicated MUC16CAR construct were co-cultured (E:T=1:2) overnight with MUC16 pos (HeLa, OVCAR3) or MUC16 neg (HEK) cell lines, with effector-only (E-only) condition used as a baseline. CAR reactivity was assessed on the basis of GFP expression, N=3 independent experiments, data are mean±SD. (E) As in (C) using primary T cells from N=3–4 healthy donors. (F) Functional activity of primary T cells incubated with the indicated target cells (top: HEK, middle: HeLa, bottom: OVCAR3). Left— Degranulation activity: CAR-transduced T cells were co-cultured with target cells at E:T=1:2 overnight, in the presence of anti-CD107a fluorescent antibody. Target cells were gated out and CD107a was detected on T cells. Data are mean±SD, n=2–4 donors, N=4–6 independent experiments, one-way ANOVA, *p<0.05, **p<0.01. Right —Cytotoxic activity: CAR T cells were tested in bioluminescence-based citotoxicity co-cultures at different E:T ratios, as indicated. Anti-CD19 CAR, FMC63, was included as an irrelevant CAR control. The time point displayed is at 10 hours of co-culture. Data are mean±SD, N=3 independent experiments, two-way ANOVAs with Dunnett’s multiple comparisons, **p<0.01, ***p<0.001. (G) Cytotoxic assay in three-dimensional (3D) cultures (spheroids). Left —Representation of the assay. Right —HeLa and HEK cells were established as spheroids for 5 days. T cells were added, and killing was measured 24 hours later (day 6). Annexin V was recorded in IncuCyte live-cell imaging, and fold increase relative to target cells alone was calculated. Data are mean of normalized values±SD, N=2 different donors, one-way ANOVA, *p<0.05. ANOVA, analysis of variance; bioluminescence-based citotoxicity, bioluminescence-based cytotoxicity; CAR, chimeric antigen receptor; E:T, effector:target ratio; HEK, human embryonic kidney; OC, ovarian cancer; TCGA, The Cancer Genome Atlas.

    Article Snippet: As a positive control, we used an anti-CA125 antibody (clone X75, Thermo Fisher Scientific, #MA1-90039) at a dilution of 1/200.

    Techniques: Expressing, In Vitro, Gene Expression, Clinical Proteomics, Membrane, Staining, Positive Control, Control, Flow Cytometry, Construct, Cell Culture, Functional Assay, Activity Assay, Incubation, Co-Culture Assay, Live Cell Imaging

    Cytokine production of MUC16CAR T cells and reactivity against primary patient samples. (A) Cytokine quantification. CAR T cells were co-cultured for 24 hours with target cells at an E:T ratio of 1:2, and the supernatant was collected for Bioplex cytokine assays. Selected cytokines are shown, data are mean±SD, N=3–4 independent measurements, two-way ANOVA. *p<0.05, **p<0.01. (B) Reactivity of CAR T cells against CA125-positive and CAR-negative samples. Tumor samples from patients with OC (effusions or material from debulking surgery) were labeled for CA125 (X75 antibody). Samples were categorized as CA125-positive (n>9), or CA125-negative (n>7), based on the percentage of cells labeling for CA125 (threshold 10%). These samples were co-cultured with CAR T cells overnight at E:T=1:2 in the presence of anti-CD107a. Effector cells were distinguished by prelabeling CAR T cells with Cell Trace Violet. CAR T cells cultured alone were used for normalization. Data are presented as mean±SD, one-way ANOVA. *p<0.05, **p<0.01. ANOVA, analysis of variance; CA125, cancer antigen 125; CAR, chimeric antigen receptor; E:T, effector:target ratio; HEK, human embryonic kidney; IL, interleukin.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Efficient CAR T cell targeting of the CA125 extracellular repeat domain of MUC16

    doi: 10.1136/jitc-2023-008179

    Figure Lengend Snippet: Cytokine production of MUC16CAR T cells and reactivity against primary patient samples. (A) Cytokine quantification. CAR T cells were co-cultured for 24 hours with target cells at an E:T ratio of 1:2, and the supernatant was collected for Bioplex cytokine assays. Selected cytokines are shown, data are mean±SD, N=3–4 independent measurements, two-way ANOVA. *p<0.05, **p<0.01. (B) Reactivity of CAR T cells against CA125-positive and CAR-negative samples. Tumor samples from patients with OC (effusions or material from debulking surgery) were labeled for CA125 (X75 antibody). Samples were categorized as CA125-positive (n>9), or CA125-negative (n>7), based on the percentage of cells labeling for CA125 (threshold 10%). These samples were co-cultured with CAR T cells overnight at E:T=1:2 in the presence of anti-CD107a. Effector cells were distinguished by prelabeling CAR T cells with Cell Trace Violet. CAR T cells cultured alone were used for normalization. Data are presented as mean±SD, one-way ANOVA. *p<0.05, **p<0.01. ANOVA, analysis of variance; CA125, cancer antigen 125; CAR, chimeric antigen receptor; E:T, effector:target ratio; HEK, human embryonic kidney; IL, interleukin.

    Article Snippet: As a positive control, we used an anti-CA125 antibody (clone X75, Thermo Fisher Scientific, #MA1-90039) at a dilution of 1/200.

    Techniques: Cell Culture, Labeling

    MUC16CAR T cell activity is unaffected by soluble antigen. Bioluminescence-based cytotoxicity assays in the presence of recombinant CA125 (300 kU/L) were performed at an E:T ratio of 10:1. Data are mean±SD, N=5 donors, one-way analysis of variance, with Šidák’s multiple comparisons test. ****p<0.0001. CA125, cancer antigen 125; CAR, chimeric antigen receptor; E:T, effector:target ratio; HEK, human embryonic kidney; ns, not significant.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Efficient CAR T cell targeting of the CA125 extracellular repeat domain of MUC16

    doi: 10.1136/jitc-2023-008179

    Figure Lengend Snippet: MUC16CAR T cell activity is unaffected by soluble antigen. Bioluminescence-based cytotoxicity assays in the presence of recombinant CA125 (300 kU/L) were performed at an E:T ratio of 10:1. Data are mean±SD, N=5 donors, one-way analysis of variance, with Šidák’s multiple comparisons test. ****p<0.0001. CA125, cancer antigen 125; CAR, chimeric antigen receptor; E:T, effector:target ratio; HEK, human embryonic kidney; ns, not significant.

    Article Snippet: As a positive control, we used an anti-CA125 antibody (clone X75, Thermo Fisher Scientific, #MA1-90039) at a dilution of 1/200.

    Techniques: Activity Assay, Recombinant